John Velazquez leads Medina Spirit to win the 147th Kentucky Derby at Churchill Downs.

Jamie Rhodes | USA TODAY Sports | Reuters

A second blood test by Kentucky Derby winner Medina Spirit has confirmed the presence of the banned steroid betamethasone, an attorney for the horse owner told CNBC on Wednesday.

The second positive test increases the risk that the Medina Spirit win will be undone for Kentucky race officials.

But Clark Brewster, the horse owner’s attorney, said officials are allowing the Medina Spirit team to have a third sample of the horse analyzed by another laboratory to see if there are any chemicals that could suspect trainer Bob Baffert’s claim would support that the betamethasone came from an ointment and not an injection.

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